Maduro asked deputy head of mission Brian Naranjo and USA charge d'affaires Todd Robinson to leave the country in 48 hours while declaring them "persona non grata".
He specifically spoke about U.S. diplomat's involvement in the conspiracies against the country saying, "We will present evidence to the country of the conspiracy in the military field of the United States charge d'affaires and his embassy, of the conspiracy in the economic field and of the conspiracy in the political field".
The U.S. State Department rejected Maduro's "false allegations" against the two diplomats, spokeswoman Heather Nauert said at a news briefing in Washington.
Robinson gave a brief speech at a public appearance on Tuesday afternoon in Venezuela's western city of Merida. It called Washington's move as part of "a systematic campaign of aggression" by USA administration and said they had no legal base.
Robinson has only been in the job at the U.S. Embassy in Caracas for a few months.
Mr. Robinson, the chargé d'affaires ordered expelled Tuesday, is a career diplomat who arrived in Caracas last December after serving as the United States ambassador to Guatemala.
"This is my first visit but it won't be my last visit to Merida or to Venezuela", he said.
Previous sanctions were limited to assets linked to individual Maduro administration members.
British-Iranian Nazanin Zaghari-Ratcliffe facing new charges
The Free Nazanin Campaign also said she was allowed to phone the British embassy in Tehran for the first time in two years. The prime minister's spokesman said the government was urgently seeking more information from Iranian authorities.
"The so-called Lima Group that has recalled (its) ambassadors is actually an anti-Venezuelan bloc cobbled together in Peru's capital at the April Summit of the Americas", the expert pointed out.
It appears to target in part Citgo [PDVSAC.UL], a USA -based oil refiner owned by Venezuela state oil company PDVSA [PDVSA.UL].
"I say to the Government of Donald Trump, I say to the government of the Ku Klux Klan", Maduro declared to applause from supporters.
Robinson said nobody in the Venezuelan government would met with him, but socialist party boss Diosdado Cabello denied the snub, accusing the diplomat of staging a "show".
The vote was marred by an opposition boycott and allegations of fraud, which led the United States to tighten sanctions.
He phoned Maduro during the night to pass on his congratulations, a Turkish presidential source was quoted as saying by state-run Anadolu news agency. Oil production has plummeted 30 percent and Venezuela has defaulted on about $50 billion in debt.
"The administration has never seen a kleptocracy like this", a senior administration official said on Monday. According to data released by Venezuela's opposition leaders leading up to the election, the cost of basic goods in the country are increasing at a rate of almost 9,000 per cent annually, and the economy shrank by a whopping 13.2 per cent in 2017.
But they appeared unlikely to heed the US warnings.